Weidenfeld J, Siegel R A, Conforti N, Feldman S, Chowers I
Endocrinology. 1981 Jul;109(1):205-9. doi: 10.1210/endo-109-1-205.
Adult male rats were given single sc injections of indomethacin (IM; 5 mg/100 g BW) and sacrificed 2-24 h later. IM effects upon serum ACTH and corticosterone (CS) levels, rectal temperature, and hypothalamic and adenohypophyseal prostaglandin E2 (PGE2) and cAMP contents were observed. Rectal temperature was normal for 5-10 h post injection and later decreased by approximately 2.5 C. Both hypothalamic and adenohypophyseal PGE2 concentrations were reduced from 2-24 h after IM administration; no changes in cAMP content were observed. Serum ACTH and CS levels were elevated 4- and 6-fold, respectively, over the entire period observed. In animals with complete hypothalamic deafferentations, the ACTH and CS responses to IM were as marked as they were in intact rats. In rats with hypothalamic lesions in which the ACTH and CS responses to ether stress were attenuated, marked ACTH and CS secretory responses to IM were seen. It is concluded: 1) that the main site of action of systemically administered IM on the hypothalamohypophyseal-adrenal axis, is within the adenohypophysis; 2) that this effect is mediated by PGE2, and cAMP is not involved; and 3) that central nervous system PGs may be involved in the maintenance of basal body temperature in the rat.
成年雄性大鼠单次皮下注射吲哚美辛（IM；5毫克/100克体重），并于2至24小时后处死。观察IM对血清促肾上腺皮质激素（ACTH）和皮质酮（CS）水平、直肠温度以及下丘脑和腺垂体前列腺素E2（PGE2）和环磷酸腺苷（cAMP）含量的影响。注射后5至10小时直肠温度正常，随后下降约2.5摄氏度。IM给药后2至24小时，下丘脑和腺垂体的PGE2浓度均降低；未观察到cAMP含量的变化。在整个观察期内，血清ACTH和CS水平分别升高了4倍和6倍。在完全去下丘脑传入神经的动物中，ACTH和CS对IM的反应与完整大鼠一样明显。在对乙醚应激的ACTH和CS反应减弱的下丘脑损伤大鼠中，也观察到了对IM明显的ACTH和CS分泌反应。得出以下结论：1）全身给药的IM对下丘脑-垂体-肾上腺轴的主要作用部位在腺垂体；2）这种作用是由PGE2介导的，与cAMP无关；3）中枢神经系统前列腺素可能参与大鼠基础体温的维持。
