Weidenfeld J, Siegel R A, Conforti N, Chowers I
Neuroendocrinology. 1980 Aug;31(2):81-4. doi: 10.1159/000123054.
The effects of the prostaglandin (PG) synthetase inhibitor indomethacin (IM) on basal and stress-induced activity of the hypothalamo-hypophyseal-adrenal (HHA) axis were investigated. IM was systemically administered to adult male rats in a single injection (5 mg/100 g BW s.c.). The animals were sacrificed 24 h later, either under basal conditions, or following 30 min exposure to environmental temperature of 36 degrees C. Serum ACTH and corticosterone (CS) were determined by RIA and CBG, respectively, and hypothalamic and adenohypophyseal PGE2 and total PGE concentrations were estimated by RIA. IM treatment resulted in elevated basal levels of both hormones. Heat exposure led to elevated serum ACTH and CS concentrations in both vehicle- and IM-treated groups, with the ACTH heat response in IM-treated animals being greater than that observed in controls. PGE2, and PGE1 plus PGE2 concentrations were markedly reduced in both hypothalamic and adenohypophyseal tissues, following IM treatment. This study demonstrates that simultaneous reductions in hypothalamic and adenohypophyseal PG concentrations are compatible with hyperactivity of the HHA axis.
研究了前列腺素（PG）合成酶抑制剂吲哚美辛（IM）对下丘脑-垂体-肾上腺（HHA）轴基础活性和应激诱导活性的影响。将IM以单次注射（5mg/100g体重，皮下注射）的方式系统地给予成年雄性大鼠。24小时后，在基础条件下或在暴露于36℃环境温度30分钟后处死动物。分别通过放射免疫分析（RIA）和皮质类固醇结合球蛋白（CBG）测定血清促肾上腺皮质激素（ACTH）和皮质酮（CS），并通过RIA估计下丘脑和腺垂体中的前列腺素E2（PGE2）和总前列腺素（PGE）浓度。IM处理导致两种激素的基础水平升高。热暴露导致载体处理组和IM处理组的血清ACTH和CS浓度升高，IM处理动物的ACTH热反应大于对照组。IM处理后，下丘脑和腺垂体组织中的PGE2以及PGE1加PGE2浓度均显著降低。本研究表明，下丘脑和腺垂体PG浓度的同时降低与HHA轴的活性亢进是相容的。
