Folbergrová J, Nilsson B, Sakabe T, Siesjö B K
J Neurochem. 1981 May;36(5):1670-4. doi: 10.1111/j.1471-4159.1981.tb00417.x.
In order to study the influence of hypoxia on cyclic nucleotides in the brain, we reduced arterial Po(2) for 15-30 min in lightly anaesthetised and artificially ventilated rats to obtain values ranging from about 45 to about 10 mm Hg. In an additional group (arterial Po(2) 18-22 mm Hg), the tissue hypoxia was aggravated by moderate arterial hypotension (mean arterial blood pressure about 80 mm Hg). In all animals, electrocortical activity was recorded. Cyclic GMP concentrations in cerebral cortex were unchanged in all groups but one. In that group, in which tissue hypoxia was severe enough to induce a suppression-burst EEG pattern and a measurable reduction in the adenylate energy charge, cyclic GMP concentrations were slightly increased (p less than 0.05). Cyclic AMP concentrations remained unaltered at all degrees of hypoxia studied. It is concluded that changes in cyclic nucleotides in brain tissue occur first at such severe degrees of hypoxia of the duration studied that function and metabolism are profoundly altered.
为了研究缺氧对脑组织中环核苷酸的影响，我们对轻度麻醉并人工通气的大鼠降低动脉血氧分压（Po₂）15 - 30分钟，以使血氧分压值在约45至约10毫米汞柱之间。在另一组实验中（动脉血氧分压为18 - 22毫米汞柱），通过中度动脉低血压（平均动脉血压约80毫米汞柱）加重组织缺氧。对所有动物记录其皮层电活动。除一组外，所有组大脑皮层中的环磷酸鸟苷（cGMP）浓度均未改变。在该组中，组织缺氧严重到足以诱发抑制 - 爆发脑电图模式以及腺苷酸能荷出现可测的降低，此时环磷酸鸟苷浓度略有升高（p < 0.05）。在所研究的所有缺氧程度下，环磷酸腺苷（cAMP）浓度均保持不变。结论是，在所研究的持续时间内，脑组织中环核苷酸的变化首先出现在如此严重的缺氧程度时，此时功能和代谢会发生深刻改变。
