感染后期从CV1细胞中提取的天然猿猴病毒40微小染色体转录的定量分析。
Quantitation of transcribing native simian virus 40 minichromosomes extracted from CV1 cells late in infection.
作者信息
Llopis R, Perrin F, Bellard F, Gariglio P
出版信息
J Virol. 1981 Apr;38(1):82-90. doi: 10.1128/JVI.38.1.82-90.1981.
Abstract
Simian virus 40 transcriptional complexes could be extracted from CV1 cells late in infection and separated from the bulk of inactive viral chromatin. Sucrose gradient sedimentation, cesium sulfate equilibrium density gradient centrifugation, and electron microscopy indicated that the viral transcriptional complexes corresponded to at least 0.5 to 1% of the viral minichromosomes extracted, about 5,000 copies per cell.
摘要
猿猴病毒40转录复合物可在感染后期从CV1细胞中提取出来，并与大部分无活性的病毒染色质分离。蔗糖梯度沉降、硫酸铯平衡密度梯度离心和电子显微镜观察表明，病毒转录复合物至少相当于所提取病毒微型染色体的0.5%至1%，即每个细胞约5000个拷贝。
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