Hoechst 33258对人白细胞的体外作用。

Effects of Hoechst 33258 on human leukocytes in vitro.

作者信息

Pimpinelli S, Prantera G, Rocchi A, Gatti M

出版信息

Cytogenet Cell Genet. 1976;17(3):114-21. doi: 10.1159/000130703.

Abstract

The benzimidazole derivative Hoechst 33258 was added at various concentrations to human leukocyte cultures. After 16 or 24 h of treatment, with concentrations equal to or greater than 100 mug/ml of Hoechst 33258, a number of chromosomes showed regions in which the chromatin was undercontracted. The centromeric regions of chromosome 1 and, more rarely, of chromosomes 3 and 9 appeared to be decondensed. Short decondensed regions were also present on the long arms of chromosomes 1 and 2. The possible nature of these regions is discussed.

摘要

将不同浓度的苯并咪唑衍生物Hoechst 33258添加到人类白细胞培养物中。在处理16或24小时后，当Hoechst 33258浓度等于或大于100微克/毫升时，一些染色体显示出染色质收缩不足的区域。1号染色体的着丝粒区域，以及较少见的3号和9号染色体的着丝粒区域似乎发生了解聚。1号和2号染色体的长臂上也存在短的解聚区域。本文讨论了这些区域可能的性质。