荧光染料Hoechst 33258对小鼠-人细胞杂交体中人类Y染色体凝聚的抑制作用。

Inhibition of condensation of human Y chromosome by the fluorochrome Hoechst 33258 in a mouse-human cell hybrid.

作者信息

Marcus M, Nattenberg A, Goitein R, Nielsén K, Gropp A

出版信息

Hum Genet. 1979 Jan 25;46(2):193-8. doi: 10.1007/BF00291921.

DOI:10.1007/BF00291921

Abstract

The fluorochrome Hoechst 33258 which binds preferentially to A-T base pairs, drastically inhibits the condensation of A-T-rich centromeric heterochromatin regions in mouse cell lines. The condensation of all other regions of these chromosomes is also inhibited to some extent. The human Y chromosome contains a large heterochromatic region, which is also rich in A-T base pairs. This chromosome is not affected by Hoechst 33258 in human leukocyte cell cultures. On the other hand, condensation of the multiple copies of human Y chromosome in the mouse-human cell hybrid RH-28Y-23 is inhibited and the chromosomes appear distorted in Hoechst 33258-treated cells.

摘要

优先与A-T碱基对结合的荧光染料Hoechst 33258能显著抑制小鼠细胞系中富含A-T的着丝粒异染色质区域的凝聚。这些染色体所有其他区域的凝聚也会受到一定程度的抑制。人类Y染色体包含一个大的异染色质区域，该区域同样富含A-T碱基对。在人类白细胞细胞培养物中，这条染色体不受Hoechst 33258的影响。另一方面，在小鼠-人类细胞杂交体RH-28Y-23中，人类Y染色体多拷贝的凝聚受到抑制，并且在经Hoechst 33258处理的细胞中，染色体出现畸变。