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伴清蛋白TATA框中的A到T碱基替换和小片段缺失显著降低了体外特异性转录。

A T to A base substitution and small deletions in the conalbumin TATA box drastically decrease specific in vitro transcription.

作者信息

Wasylyk B, Chambon P

出版信息

Nucleic Acids Res. 1981 Apr 24;9(8):1813-24. doi: 10.1093/nar/9.8.1813.

DOI:10.1093/nar/9.8.1813
PMID:6264394
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC326805/
Abstract

We have previously shown that a T to G transversion at the second T of the conalbumin "TATA" box drastically decreases specific initiation of transcription by RNA polymerase B (1). We now report that a T to A transcription at the same nucleotide also drastically decreases transcription, suggesting that these mutations influence transcription mainly by altering nucleotide recognition rather than helix stability. Small deletions of 2, 3 and 4 nucleotides in the "TATA" box also drastically decrease specific initiation of transcription.

摘要

我们之前已经表明，伴清蛋白“TATA”框第二个T处的T到G颠换会大幅降低RNA聚合酶B的特异性转录起始（1）。我们现在报告，同一核苷酸处的T到A转录也会大幅降低转录，这表明这些突变主要通过改变核苷酸识别而非螺旋稳定性来影响转录。“TATA”框中2、3和4个核苷酸的小缺失也会大幅降低转录的特异性起始。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae8f/326805/b3acdfa11add/nar00401-0047-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae8f/326805/901efe74e8d9/nar00401-0046-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae8f/326805/b3acdfa11add/nar00401-0047-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae8f/326805/901efe74e8d9/nar00401-0046-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae8f/326805/b3acdfa11add/nar00401-0047-a.jpg

相似文献

1
A T to A base substitution and small deletions in the conalbumin TATA box drastically decrease specific in vitro transcription.伴清蛋白TATA框中的A到T碱基替换和小片段缺失显著降低了体外特异性转录。
Nucleic Acids Res. 1981 Apr 24;9(8):1813-24. doi: 10.1093/nar/9.8.1813.
2
Specific in vitro transcription of conalbumin gene is drastically decreased by single-point mutation in T-A-T-A box homology sequence.伴清蛋白基因的特异性体外转录因T-A-T-A盒同源序列中的单点突变而急剧减少。
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7024-8. doi: 10.1073/pnas.77.12.7024.
3
Specific in vitro initiation of transcription on conalbumin and ovalbumin genes and comparison with adenovirus-2 early and late genes.伴清蛋白和卵清蛋白基因转录的特异性体外起始及与腺病毒2型早期和晚期基因的比较。
Nature. 1980 Jun 5;285(5764):367-73. doi: 10.1038/285367a0.
4
The conalbumin "TATA" box sequence and the SV40 72 base-pair repeat region influence expression of a chimeric gene in vivo.伴清蛋白“TATA”盒序列和SV40 72碱基对重复区域影响嵌合基因在体内的表达。
Prog Clin Biol Res. 1982;85 Pt A:13-24.
5
Organization and sequence studies of the 17-piece chicken conalbumin gene.17片段鸡伴白蛋白基因的组织与序列研究
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6
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Promotion of specific in vitro transcription by excised "TATA" box sequences inserted in a foreign nucleotide environment.插入外源核苷酸环境中的切除“TATA”框序列对特定体外转录的促进作用。
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引用本文的文献

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Plant Mol Biol. 1986 Jan;7(1):71-9. doi: 10.1007/BF00020133.
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Hepatitis B virus with mutations in the core promoter for an e antigen-negative phenotype in carriers with antibody to e antigen.在e抗原抗体携带者中具有核心启动子突变的乙型肝炎病毒，表现为e抗原阴性表型。
J Virol. 1994 Dec;68(12):8102-10. doi: 10.1128/JVI.68.12.8102-8110.1994.
3
Promotion of specific in vitro transcription by excised "TATA" box sequences inserted in a foreign nucleotide environment.

本文引用的文献

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The effect of bisulfite modification on the template activity of DNA for DNA polymerase I.亚硫酸氢盐修饰对DNA聚合酶I模板活性的影响。
Nucleic Acids Res. 1974 Jul;1(7):889-99. doi: 10.1093/nar/1.7.889.
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Ubiquitous and gene-specific regulatory 5' sequences in a sea urchin histone DNA clone coding for histone protein variants.一个编码组蛋白变体的海胆组蛋白DNA克隆中普遍存在的和基因特异性的调控5'序列。
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插入外源核苷酸环境中的切除“TATA”框序列对特定体外转录的促进作用。
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The yeast his3 promoter contains at least two distinct elements.酵母his3启动子至少包含两个不同的元件。
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Simian virus 40 major late promoter: an upstream DNA sequence required for efficient in vitro transcription.猴病毒40主要晚期启动子：体外高效转录所需的上游DNA序列。
Mol Cell Biol. 1984 Jan;4(1):133-41. doi: 10.1128/mcb.4.1.133-141.1984.
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Determination of the promoter region of mouse ribosomal RNA gene by an in vitro transcription system.利用体外转录系统确定小鼠核糖体RNA基因的启动子区域。
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Introduction of restriction enzyme sites in protein-coding DNA sequences by site-specific mutagenesis not affecting the amino acid sequence: a computer program.通过不影响氨基酸序列的位点特异性诱变在蛋白质编码DNA序列中引入限制性酶切位点：一个计算机程序
Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):777-87. doi: 10.1093/nar/12.1part2.777.
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Nucleic Acids Res. 1983 Jul 25;11(14):4727-34. doi: 10.1093/nar/11.14.4727.
注射到非洲爪蟾卵母细胞核中的组蛋白基因的转录保真度。
Nature. 1980 Nov 6;288(5786):100-2. doi: 10.1038/288100a0.
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Accurate transcription initiation on a purified mouse beta-globin DNA fragment in a cell-free system.在无细胞体系中，在纯化的小鼠β-珠蛋白DNA片段上进行准确的转录起始。
Cell. 1980 Jul;20(3):691-9. doi: 10.1016/0092-8674(80)90315-3.
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Specific in vitro initiation of transcription on conalbumin and ovalbumin genes and comparison with adenovirus-2 early and late genes.伴清蛋白和卵清蛋白基因转录的特异性体外起始及与腺病毒2型早期和晚期基因的比较。
Nature. 1980 Jun 5;285(5764):367-73. doi: 10.1038/285367a0.
6
DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract.腺病毒基因在可溶性全细胞提取物中的DNA依赖性转录。
Proc Natl Acad Sci U S A. 1980 Jul;77(7):3855-9. doi: 10.1073/pnas.77.7.3855.
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Proc Natl Acad Sci U S A. 1980 Mar;77(3):1432-6. doi: 10.1073/pnas.77.3.1432.
8
Regulation of simian virus 40 early transcription in vitro by a purified tumor antigen.一种纯化的肿瘤抗原对猿猴病毒40早期转录的体外调控
Proc Natl Acad Sci U S A. 1980 Oct;77(10):5706-10. doi: 10.1073/pnas.77.10.5706.
9
Promoter sequences of eukaryotic protein-coding genes.真核生物蛋白质编码基因的启动子序列。
Science. 1980 Sep 19;209(4463):1406-14. doi: 10.1126/science.6251548.
10
E. coli RNA polymerase interacts homologously with two different promoters.大肠杆菌RNA聚合酶与两种不同的启动子进行同源性相互作用。
Cell. 1980 Jun;20(2):269-81. doi: 10.1016/0092-8674(80)90613-3.