Ghosh P K, Lebowitz P, Frisque R J, Gluzman Y
Proc Natl Acad Sci U S A. 1981 Jan;78(1):100-4. doi: 10.1073/pnas.78.1.100.
The 5' termini of the principal early mRNAs produced in cells transformed by wild-type simian virus 40 lie 21-25 nucleotides downstream from an A-T-T-T-A-T sequence on the DNA template. The 5' termini of early mRNAs produced by five origin-defective mutants containing deletions downstream from the A-T-T-T-A-T sequence and one viable mutant dl892 with a deletion starting 15 nucleotides upstream from this sequence were determined by a method involving synthesis, separation, and determination of the sequences of DNAs complementary to 5' termini. Mutant dl892 produced early mRNAs with the same principal 5' termini as wild-type virus; the origin-defective mutants produced mRNAs with principal 5' termini shifted downstream by a distance equivalent to the length of the deleted DNA segment. These data suggest that a DNA sequence of 29 nucleotides, which includes the A-T-T-T-A-T sequence, contains a component(s) of a promoter for early transcription. This component functions in positioning the 5' ends of the principal early mRNAs 21-25 nucleotides downstream from the A-T-T-T-A-T sequence and acts independently of these downstream sequences.
在被野生型猴病毒40转化的细胞中产生的主要早期mRNA的5'末端，位于DNA模板上A-T-T-T-A-T序列下游21-25个核苷酸处。通过一种涉及合成、分离和测定与5'末端互补的DNA序列的方法，确定了五个起源缺陷型突变体产生的早期mRNA的5'末端，这些突变体在A-T-T-T-A-T序列下游含有缺失，以及一个存活突变体dl892，其缺失从该序列上游15个核苷酸处开始。突变体dl892产生的早期mRNA的主要5'末端与野生型病毒相同；起源缺陷型突变体产生的mRNA的主要5'末端向下游移动了一段相当于缺失DNA片段长度的距离。这些数据表明，一个包含A-T-T-T-A-T序列的29个核苷酸的DNA序列包含早期转录启动子的一个成分。该成分在将主要早期mRNA的5'末端定位在A-T-T-T-A-T序列下游21-25个核苷酸处起作用，并且独立于这些下游序列发挥作用。
