Keene M A, Corces V, Lowenhaupt K, Elgin S C
Proc Natl Acad Sci U S A. 1981 Jan;78(1):143-6. doi: 10.1073/pnas.78.1.143.
By using a map of the unique region of DNA encoding the fur small heat-shock proteins of Drosophila melanogaster (hsp 22, hsp 23, hsp 26, and hsp 28), and a simple mapping technique, the positions of the DNase I hypersensitive sites of chromatin in the vicinity of these genes have now been determined. The major chromatin-specific sites occur at the 5' ends of each of the four heat-shock protein genes in embryo nuclei. These genes are not active in the nuclei analyzed but can be quickly induced in these cells by the heat-shock stimulus. The chromatin structure indicated by DNase I hypersensitivity may be a necessary factor in the general mechanism of gene activation.
通过使用一幅编码黑腹果蝇（hsp 22、hsp 23、hsp 26和hsp 28）的fur小热休克蛋白的独特DNA区域图谱，以及一种简单的定位技术，现已确定了这些基因附近染色质的DNase I超敏位点的位置。主要的染色质特异性位点出现在胚胎细胞核中四个热休克蛋白基因各自的5'端。这些基因在所分析的细胞核中不活跃，但可通过热休克刺激在这些细胞中快速诱导激活。DNase I超敏性所表明的染色质结构可能是基因激活一般机制中的一个必要因素。
