Tax A, Manson L A
Proc Natl Acad Sci U S A. 1981 Jan;78(1):529-33. doi: 10.1073/pnas.78.1.529.
We have produced lymphocyte hybridomas between mouse myeloma cells and either spleen cells of C3H/f/C57BL/6 mice bearing the Mm5mt/c1 tumor-producing murine mammary tumor virus (MMTV) or spleen cells from Fisher rats inoculated with the same tumor. Two classes of hybridoma-secreted monoclonal antibodies were obtained. In the first class are IVC11, IIIA1, and VE7, each of which precipitated a 52,000-dalton protein from 125I-labeled purified preparations of MMTV and [3H]glucosamine-labeled Mm5mt/c1 cell extracts. A second class of monoclonal antibodies, represented by IC12 and IIIB5, reacted specifically with C3H MMTV-secreting cells in radioimmunoassays (RIA) with whole cells and did not precipitate proteins with labeled virus or metabolically labeled Mm5mt/c cell extracts. IC12 and IIB5 could be distinguished from each other in a RIA in which antigen discs were prepared from a membrane preparation of Mm5mt/c1 cells. In this assay, IC12 antibody was blocked from reacting with discs precoated with goat anti-MMTV, and IIB5 antibody was not.
我们已在小鼠骨髓瘤细胞与携带Mm5mt/c1肿瘤的C3H/f/C57BL/6小鼠的脾细胞（该肿瘤产生鼠乳腺肿瘤病毒（MMTV））或接种了相同肿瘤的Fisher大鼠的脾细胞之间制备了淋巴细胞杂交瘤。获得了两类杂交瘤分泌的单克隆抗体。第一类包括IVC11、IIIA1和VE7，它们每一个都能从125I标记的纯化MMTV制剂和[3H]葡糖胺标记的Mm5mt/c1细胞提取物中沉淀出一种52,000道尔顿的蛋白质。第二类单克隆抗体以IC12和IIIB5为代表，在全细胞放射免疫测定（RIA）中与分泌C3H MMTV的细胞特异性反应，并且不会用标记病毒或代谢标记的Mm5mt/c细胞提取物沉淀蛋白质。在一种RIA中，IC12和IIB5可以相互区分，在该RIA中，抗原盘由Mm5mt/c1细胞的膜制剂制备。在该测定中，IC12抗体与预涂有山羊抗MMTV的盘反应被阻断，而IIB5抗体则未被阻断。
