Ihle J N, Arthur L O, Fine D L
Cancer Res. 1976 Aug;36(8):2840-4.
Specific radioimmune precipitation assays were utilized to demonstrate the presence of precipitating antibodies to mouse mammary tumor virus (MMTV) in the high-spontaneous mammary tumor strains of mice: C3H/HeN+, GR/N, BALB/cfC3H, and C57BL/6 X C3H F1 (hereafter called B6C3F1). Antibody titers in C3H/HeN+ mice increased with age, with highest titers observed in tumor-bearing animals. MMTV-precipitating antibodies were not detectable by radioimmune precipitation assay in low-mammary tumor strains (AKR, BALB/c C57BL/6, and C3H/HeN-) but were detectable in MMTV-inoculated BALB/c mice. Appearance of antibodies preceded palpable tumor formation, and antibody titers were directly correlated to virus dose. Natural antibody to MMTV in C3H/HeN+ and B6C3F1 mice coexists with the murine leukemia virus natural antibody as determined by competition radioimmunoassays.
利用特异性放射免疫沉淀试验来证明在高自发性乳腺肿瘤小鼠品系（C3H/HeN+、GR/N、BALB/cfC3H和C57BL/6×C3H F1，以下称为B6C3F1）中存在针对小鼠乳腺肿瘤病毒（MMTV）的沉淀抗体。C3H/HeN+小鼠中的抗体滴度随年龄增加，在患肿瘤动物中观察到最高滴度。在低乳腺肿瘤品系（AKR、BALB/c C57BL/6和C3H/HeN-）中，通过放射免疫沉淀试验无法检测到MMTV沉淀抗体，但在接种MMTV的BALB/c小鼠中可以检测到。抗体的出现先于可触及肿瘤的形成，并且抗体滴度与病毒剂量直接相关。通过竞争放射免疫测定法确定，C3H/HeN+和B6C3F1小鼠中针对MMTV的天然抗体与鼠白血病病毒天然抗体共存。
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