胎儿三毛滴虫中硝基咪唑抗性的检测条件及验证
Assay conditions and the demonstration of nitroimidazole resistance in Tritrichomonas foetus.
作者信息
Meingassner J G, Mieth H, Czok R, Lindmark D G, Müller M
出版信息
Antimicrob Agents Chemother. 1978 Jan;13(1):1-3. doi: 10.1128/AAC.13.1.1.
Abstract
Tritrichomonas foetus KV(1), a nitroimidazole-susceptible strain, and KV(1)/M100, its nitroimidazole-resistant daughter strain, differed markedly in their in vivo susceptibility to metronidazole. In vitro susceptibility testing in multiwell plates and tubes with different trichomonad media containing no, or low concentrations of, ascorbate demonstrated that the resistant strain behaves like the susceptible one, if tested under anaerobic conditions (deep cultures in tubes or multiwell plates in anaerobic jars), but shows resistance if tested in the presence of air (multiwell plates exposed to air). In media containing high concentrations of ascorbate, no resistance was observed even in air. The results suggest that the two strains differ in the regulation of internal redox systems and underscore the role testing methods may play in the in vitro detection of nitroimidazole-resistant protozoan parasites.
摘要
胎儿三毛滴虫KV(1)是一株对硝基咪唑敏感的菌株，其耐硝基咪唑的子代菌株KV(1)/M100在体内对甲硝唑的敏感性存在显著差异。在含有无或低浓度抗坏血酸的不同滴虫培养基的多孔板和试管中进行体外药敏试验表明，如果在厌氧条件下（试管中的深层培养或厌氧罐中的多孔板）进行测试，耐药菌株的表现与敏感菌株相似，但在有空气存在的情况下（暴露于空气中的多孔板）进行测试则显示出耐药性。在含有高浓度抗坏血酸的培养基中，即使在空气中也未观察到耐药性。结果表明，这两种菌株在内部氧化还原系统的调节方面存在差异，并强调了检测方法在体外检测耐硝基咪唑原生动物寄生虫中可能发挥的作用。
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