Danes L, Cerva L
J Hyg Epidemiol Microbiol Immunol. 1981;25(2):169-74.
Cultures of Acanthamoeba castellanii, kept at room temperature in sterile Bacto-Casitone (Difco) medium, were artificially infected with vaccination poliovirus strains type 1 and type 3 and with echovirus type 4 and echovirus type 30. No remarkable virus cumulation was observed on the surface or inside amoeba cells during the observation period of 21 days. Amoeba-adsorbed viruses were impossible to remove by repeated washings. Virus neutralization with specific antisera showed that enteroviruses were most probably present only on amoeba surfaces. In contrast to amoeba-free virus suspension, echoviruses bound to amoebae and their cell pulp persisted even after 52 to 75 days. However, the tested amoeba species played only the role of a solids-like carrier in this survival of echoviruses.
将卡氏棘阿米巴培养物保存在室温下的无菌细菌蛋白胨（Difco）培养基中，用1型和3型脊髓灰质炎疫苗株以及4型和30型艾柯病毒进行人工感染。在21天的观察期内，未在阿米巴细胞表面或内部观察到明显的病毒累积。通过反复洗涤无法去除吸附在阿米巴上的病毒。用特异性抗血清进行病毒中和试验表明，肠道病毒很可能仅存在于阿米巴表面。与无阿米巴的病毒悬液相比，即使在52至75天后，与阿米巴结合的艾柯病毒及其细胞浆仍持续存在。然而，在这种艾柯病毒的存活过程中，所测试的阿米巴物种仅起到类似固体载体的作用。
