Shinomiya T, Sato S
Nucleic Acids Res. 1980 Jan 11;8(1):43-56. doi: 10.1093/nar/8.1.43.
A site specific endonuclease with novel specificity has been isolated from Thermus thermophilus strain 111 and named Tth111I. Tth111I cleaves lambda DNA into three fragments of 23.5, 25.7 and 50.8% of the total length, and ColE1 DNA into two fragments of nearly equal length. The sequences around Tth111I cleavage sites of ColE1 and lambda DNA were determined by the Maxam and Gilbert method and the two dimensional mapping method. The results suggest that Tth111I recognizes the DNA sequence (formula: see text) and cleaves the site as indicated by the arrows. Assuming that the first T.A pair in the sequence can be replaced for any base pair, the Tth111I recognition sequence has the symmetry with the two-fold axis as most type II restriction endonucleases do.
一种具有新型特异性的位点特异性内切核酸酶已从嗜热栖热菌菌株111中分离出来，并命名为Tth111I。Tth111I将λDNA切割成三个片段，分别占总长度的23.5%、25.7%和50.8%，并将ColE1 DNA切割成两个长度几乎相等的片段。通过Maxam和Gilbert方法以及二维图谱法确定了ColE1和λDNA的Tth111I切割位点周围的序列。结果表明，Tth111I识别DNA序列（公式：见文本），并按箭头所示切割位点。假设序列中的第一个T.A对可以被任何碱基对取代，Tth111I识别序列与大多数II型限制性内切核酸酶一样具有二重轴对称性。
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