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来自嗜热水生栖热菌的第二种具有异常序列特异性的II型限制性内切酶。

A second type II restriction endonuclease from Thermus aquaticus with an unusual sequence specificity.

作者信息

Barker D, Hoff M, Oliphant A, White R

出版信息

Nucleic Acids Res. 1984 Jul 25;12(14):5567-81. doi: 10.1093/nar/12.14.5567.

DOI:10.1093/nar/12.14.5567
PMID:6087291
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC320015/
Abstract

A type II restriction endonuclease activity free of TaqI was prepared from Thermus Aquaticus YT. The fraction contains two endonucleolytic components with apparently different specificities, however the major activity is sufficiently dominant to allow partial digestion analysis of the position of recognition sites. A precise determination of the location of cleavage sites in pBR322 DNA and a computer-aided search for regions of homology in the vicinity of the cut sites indicate that this enzyme recognizes the nonpalindromic sequences GACCGA or CACCCA. Other related sequences are not cleaved, in particular, GACCCA and CACCGA, indicating that the enzyme requires the identity of nucleotides in the first and fifth positions, a type of specificity that has not been previously reported. The position of cleavage is located outside of the site and is represented as: (Formula: see text).

摘要

从嗜热水生栖热菌YT中制备了一种不含TaqI的II型限制性内切核酸酶活性。该组分含有两种内切核酸酶成分，其特异性明显不同，然而主要活性足够占主导地位，以允许对识别位点的位置进行部分消化分析。对pBR322 DNA中切割位点位置的精确测定以及对切割位点附近同源区域的计算机辅助搜索表明，该酶识别非回文序列GACCGA或CACCCA。其他相关序列不被切割，特别是GACCCA和CACCGA，这表明该酶需要第一位和第五位核苷酸相同，这种特异性类型以前尚未报道。切割位置位于位点之外，其表示为：（公式：见正文）。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a25c/320015/85465fba8276/nar00332-0040-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a25c/320015/3f2be62063b2/nar00332-0036-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a25c/320015/8e33abaf4aee/nar00332-0039-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a25c/320015/85465fba8276/nar00332-0040-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a25c/320015/3f2be62063b2/nar00332-0036-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a25c/320015/8e33abaf4aee/nar00332-0039-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a25c/320015/85465fba8276/nar00332-0040-a.jpg

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