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从一只患腹泻的新生犬中分离出一株轮状病毒。

Isolation of a rotavirus from a newborn dog with diarrhea.

作者信息

Fulton R W, Johnson C A, Pearson N J, Woode G N

出版信息

Am J Vet Res. 1981 May;42(5):841-3.

PMID:6266295
Abstract

A rotavirus was isolated from a newborn dog that died after having clinical signs of diarrhea. Virus particles with rotaviral morphologic features were observed by transmission electron microscopy in the intestinal homogenate collected at necropsy. Cytopathic effects were observed, and rotaviral antigens were detected by indirect immunofluorescence in MA-104 monolayer cultures (a fetal rhesus macaque kidney cell) inoculated with intestinal homogenate. This rotavirus isolate, designated LSU 79C-36, may be a specific canine rotavirus or a rotavirus from another species.

摘要

从一只出现腹泻临床症状后死亡的新生犬中分离出一株轮状病毒。尸检时收集的肠道匀浆经透射电子显微镜观察,发现了具有轮状病毒形态特征的病毒颗粒。观察到细胞病变效应,在用肠道匀浆接种的MA-104单层培养物(恒河猴胎儿肾细胞)中通过间接免疫荧光检测到轮状病毒抗原。这株分离的轮状病毒命名为LSU 79C-36,可能是一种特定的犬轮状病毒,也可能是来自其他物种的轮状病毒。

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