Mironova G D, Fedotcheva N I, Makarov P R, Pronevich L A, Mironov G P
Biofizika. 1981 May-Jun;26(3):451-7.
Protein (M. m. 60 000) inducing selective potassium conductance of bilayer lipid membranes (BLM) was isolated from mitochondria and homogenate of the beef heart. This protein was obtained by means of alcohol (ethanol) extraction and was purified by gel-filtration on Sephadex G-15 and G-50 followed by electrophoresis in the 10% polyacrylamide gel. 6-10 g/ml of the protein produced the conductivity channels on BLM with amplitude divisible of 24 +/- 4 pmho. The channels of 175 +/- 7 pmho were the most typical ones. The modification of BLM by K+-transport in protein under the conditions of potassium gradient resulted in the appearance of the membrane potential close to the theoretical Nernst potential.
从牛心线粒体和匀浆中分离出一种能诱导双层脂质膜（BLM）产生选择性钾离子电导的蛋白质（分子量为60000道尔顿）。该蛋白质通过乙醇提取获得，并先后经Sephadex G - 15和G - 50凝胶过滤，随后在10%聚丙烯酰胺凝胶中进行电泳纯化。6 - 10微克/毫升的该蛋白质能在BLM上产生电导通道，其振幅可分为24±4皮西门子。175±7皮西门子的通道最为典型。在钾离子梯度条件下，蛋白质中钾离子转运对BLM的修饰导致膜电位的出现，接近理论能斯特电位。
