Makhmudova E M, Bespal'ko N V, Kazakov I, Krasil'nikov O V, Ternovskiĭ V
Biofizika. 1983 Jan-Feb;28(1):140-2.
Individual low molecular weight protein component was isolated by gel-filtration method from the inner mitochondrial membrane. This membrane component increases the conductivity of bilayer lipid membranes (BLM) in the presence of K+ and Ca2+-ions. This phenomenon may be explained by the formation of single conductivity channels. The voltage - current characteristics of this channel is nonlinear, which may be the result of asymmetrical operation of the channel - former in the polarized membrane, in spite of equal concentration of protein on both sides of the membrane.
通过凝胶过滤法从线粒体内膜中分离出单个低分子量蛋白质成分。这种膜成分在存在钾离子和钙离子的情况下会增加双层脂质膜(BLM)的导电性。这种现象可能是由于形成了单个导电通道所致。该通道的电压-电流特性是非线性的,尽管膜两侧蛋白质浓度相等,但这可能是通道形成者在极化膜中不对称运作的结果。