[Entry of double-and single-stranded linear DNA in Ca2+-treated Escherichia coli cells].
作者信息
Sabel'nikov A G, Bukrinskiĭ M I, Il'iashenko B N
出版信息
Mol Biol (Mosk). 1981 Mar-Apr;15(2):403-7.
PMID:7017383
Abstract
Hydroxylapatite chromatography technique was used to investigate the conformational changes undergone by exogenous linear DNA during the uptake by Ca2+-treated EScherichia coli cells. Both single- (heat denatured) and double-stranded DNAs were examined. While native DNA preserved its double-strandedness during the uptake, the heat denatured (single-stranded) DNA was rapidly converted to the double-stranded form. This process was energy-dependent and is supposed to be dependent on gene recA product.
摘要
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