Laughran L J, Larsen J H, Schroeder P C
Cell Tissue Res. 1981;218(3):537-45. doi: 10.1007/BF00210113.
Two types of filaments (microfilaments 4--6 nm in diameter, and intermediate filaments 7--10 nm in diameter) are common in the surface epithelial cells and theca fibroblasts of vitellogenic ovarian follicles of the lizard Anolis carolinensis. Heavy meromyosin (HMM), which forms complexes with actin filaments, interacts only with the microfilaments of theca fibroblasts. After myosin extraction of follicles no filaments disappeared, but when this treatment was followed by incubation with deoxyribonuclease I (DNA-ase I), which depolymerizes F-actin to G-actin, microfilaments disappeared from the theca fibroblasts. It is concluded that microfilaments in theca fibroblasts are actin-like and may contract to provide the mechanism of expulsion for the oocyte during ovulation. The intermediate filaments of the surface epithelial cells and theca fibroblasts may serve as a skeletal system for the large (up to 8 mm in diameter) vitellogenic follicle.
在绿安乐蜥卵黄生成期卵巢卵泡的表面上皮细胞和卵泡膜成纤维细胞中，常见两种类型的细丝（直径为4 - 6纳米的微丝和直径为7 - 10纳米的中间丝）。与肌动蛋白丝形成复合物的重酶解肌球蛋白（HMM）仅与卵泡膜成纤维细胞的微丝相互作用。在对卵泡进行肌球蛋白提取后，没有细丝消失，但当这种处理之后再用脱氧核糖核酸酶I（DNA酶I）孵育时，微丝从卵泡膜成纤维细胞中消失了，DNA酶I可使F - 肌动蛋白解聚为G - 肌动蛋白。由此得出结论，卵泡膜成纤维细胞中的微丝类似肌动蛋白，可能会收缩以提供排卵期间卵母细胞排出的机制。表面上皮细胞和卵泡膜成纤维细胞的中间丝可能作为大的（直径达8毫米）卵黄生成期卵泡的骨骼系统。
