Vinayak V K, Singh K, Venkatwswarlu K, Nain C K, Mehta S K
J Clin Microbiol. 1986 Jun;23(6):1088-90. doi: 10.1128/jcm.23.6.1088-1090.1986.
A micro enzyme-linked immunosorbent assay was used to detect the presence of amoebic antigen in polyethylene-glycol-precipitated circulating immune complexes (CIC). A cutoff dilution of greater than 1:160 for the precipitates was taken to be of clinical significance. Among the patients with amoebic liver abscess, 93% (14 of 15 confirmed cases) had indications of amoebic antigen in CIC. In 57% of the suspected cases of amoebic liver abscess, amoebic antigen could be detected in CIC. The specificity of the technique for the demonstration of specific amoebic antigen is indicated by the undetectable levels of amoebic antigen in CIC in serum samples from nine cases of nonamoebic hepatic diseases and 10 apparently healthy subjects. It is thus believed that the specific diagnosis of an individual case of amoebic liver abscess can be made by demonstrating specific amoebic antigen in CIC.
采用微量酶联免疫吸附测定法检测聚乙二醇沉淀的循环免疫复合物（CIC）中阿米巴抗原的存在。沉淀物的临界稀释度大于1:160被认为具有临床意义。在阿米巴肝脓肿患者中，93%（15例确诊病例中的14例）的CIC中有阿米巴抗原迹象。在57%的疑似阿米巴肝脓肿病例中，可在CIC中检测到阿米巴抗原。9例非阿米巴性肝病患者和10名明显健康受试者的血清样本中，CIC中的阿米巴抗原水平无法检测到，这表明该技术用于显示特定阿米巴抗原的特异性。因此，人们认为通过在CIC中显示特定的阿米巴抗原可以对个别阿米巴肝脓肿病例做出特异性诊断。
