Arnold W, Klein M, Wang J B, Schmidt W A, Trampisch H J
Arch Otorhinolaryngol. 1981;232(2):165-77. doi: 10.1007/BF00505035.
Coronavirus-like particles are found within the cytoplasm of NPC tumor cells, within the cytoplasm of the tumor cells of the regional metastases, and within tumor cells grown on nude mice. For the immunologic identification of the coronaviruses, the cultures of human tracheal epithelium (MRC-C) were used and inoculated with a known coronavirus strain. Whereas blood sera from NPC patients (n = 73) contain significantly elevated antibody titers against corona viruses, unselected sera from patients without NPC showed a low antibody titer (n = 83). Only patients suffering from infectious mononucleosis (n = 40) showed a titer pattern similar to that of NPC patients. For demonstration of antigen-antibody reaction within the NPC tumor cell cytoplasm, sera with a high antibody content against coronaviruses deriving from other than NPC patients or anticoronavirus sera from rabbits were used. By indirect immunofluorescence, the NPC tumor cells showed a bright cytoplasmic fluorescence. No fluorescence was seen when tumor cells were exposed to human sera with known low or absent corona antibody titer or to normal rabbit sera. The results indicate that next to a DNA virus infection (EBV), an RNA virus infection (coronavirus) may play a role in NPC as well as in infectious mononucleosis.
在鼻咽癌肿瘤细胞的细胞质内、区域转移瘤细胞的细胞质内以及在裸鼠身上生长的肿瘤细胞内均发现了冠状病毒样颗粒。为了对冠状病毒进行免疫学鉴定，使用了人气管上皮细胞培养物（MRC-C）并接种已知的冠状病毒株。鼻咽癌患者（n = 73）的血清中针对冠状病毒的抗体滴度显著升高，而未患鼻咽癌患者的未筛选血清显示抗体滴度较低（n = 83）。只有患传染性单核细胞增多症的患者（n = 40）显示出与鼻咽癌患者相似的滴度模式。为了证明鼻咽癌肿瘤细胞质内的抗原-抗体反应，使用了来自非鼻咽癌患者的具有高冠状病毒抗体含量的血清或兔抗冠状病毒血清。通过间接免疫荧光法，鼻咽癌肿瘤细胞显示出明亮的细胞质荧光。当肿瘤细胞暴露于已知冠状病毒抗体滴度低或无抗体滴度的人血清或正常兔血清时，未观察到荧光。结果表明，除了DNA病毒感染（EBV）外，RNA病毒感染（冠状病毒）可能在鼻咽癌以及传染性单核细胞增多症中也起作用。
