在鸡胚细胞中生长期间标记的传染性支气管炎病毒颗粒的结构蛋白和糖蛋白。

Structural proteins and glycoproteins of infectious bronchitis virus particles labeled during growth in chick embryo cells.

作者信息

Wadey C N, Westaway E G

出版信息

Intervirology. 1981;15(1):19-27. doi: 10.1159/000149210.

Abstract

Seven Australian infectious bronchitis viruses incorporated radioactive amino acid label during growth in two types of cell culture. From purified virions, four major polypeptides of molecular weights 91,000 (GP91), 51,000 (VP51), 25,000 (GP25) and 23,000 (VP23) were separated by PAGE. Two minor polypeptides of molecular weights 210,000 (GP210) and 185,000 (GP185) were also identified. 3H-glucosamine was incorporated during growth of virus into GP210, GP185, GP91 and GP25. Bromelain digestion of virions removed GP210, GP185 and GP91, leaving VP51, GP25 and VP23 in a large subviral particle. All seven viruses, which were of six serological subtypes, formed apparently identical polypeptide profiles.

摘要

七种澳大利亚传染性支气管炎病毒在两种细胞培养物中生长时掺入了放射性氨基酸标记。从纯化的病毒粒子中，通过聚丙烯酰胺凝胶电泳（PAGE）分离出分子量分别为91,000（GP91）、51,000（VP51）、25,000（GP25）和23,000（VP23）的四种主要多肽。还鉴定出分子量为210,000（GP210）和185,000（GP185）的两种次要多肽。在病毒生长过程中，3H-葡糖胺掺入到GP210、GP185、GP91和GP25中。用菠萝蛋白酶消化病毒粒子可去除GP210、GP185和GP91，在一个大的亚病毒颗粒中留下VP51、GP25和VP23。这七种病毒分属六种血清型，其多肽图谱明显相同。