Gallagher J S, Tse C S, Brooks S M, Bernstein I L
J Occup Med. 1981 Sep;23(9):610-6. doi: 10.1097/00043764-198109000-00009.
Possible immunoreactivity to chemically well-characterized mono- and diisocyanate protein conjugates was reevaluated in 15 workers with TDI asthma and 17 normal (nonexposed) volunteers. Lymphocytes of nine sensitive workers were incubated with TDI human serum albumin (HSA) conjugates. Leucocyte inhibitory factor (LIF) was produced. Leucocyte inhibitory factor was also induced by hexamethylene diisocyanate (HDI) protein conjugates in four of these workers who had no prior history of exposure to HDI. Disappearance of TDI- and HDI-induced LIF was noted in several sensitive workers who were removed from further TDI exposure. Three LIF-positive workers also demonstrated positive intracutaneous reactivity to TDI-HSA. One workers had a markedly positive RAST (25.5% binding) to a monofunctional (p-tolyl isocyanate) protein reagent. These studies suggest that isocyanates have the potential for eliciting heterogeneous immune responses in certain subpopulations of exposed workers. Continued contact with isocyanates may be necessary for maintenance of specific immunity. Possible cross reactivity between TDI and HDI may be determined by new antigenic sites created by isocyanate protein interactions.
在15名患有甲苯二异氰酸酯（TDI）哮喘的工人和17名正常（未接触过）志愿者中，重新评估了对化学特性明确的单异氰酸酯和二异氰酸酯蛋白缀合物的可能免疫反应性。将9名敏感工人的淋巴细胞与TDI人血清白蛋白（HSA）缀合物一起孵育。产生了白细胞抑制因子（LIF）。在这些之前没有接触过六亚甲基二异氰酸酯（HDI）的工人中，有4人也被HDI蛋白缀合物诱导产生了白细胞抑制因子。在一些不再进一步接触TDI的敏感工人中，观察到TDI和HDI诱导的LIF消失。3名LIF阳性的工人对TDI-HSA也表现出阳性皮内反应性。1名工人对一种单官能（对甲苯基异氰酸酯）蛋白试剂有明显阳性的放射性变应原吸附试验（RAST）结果（结合率为25.5%）。这些研究表明，异氰酸酯有可能在接触工人的某些亚群中引发异质性免疫反应。持续接触异氰酸酯可能是维持特异性免疫所必需的。TDI和HDI之间可能的交叉反应性可能由异氰酸酯与蛋白相互作用产生的新抗原位点决定。
