Minor P D
J Virol. 1980 Apr;34(1):73-84. doi: 10.1128/JVI.34.1.73-84.1980.
A study of the biochemistry of type 3 poliovirus strains which involves the examination of the virus-coded polypeptides in infected cells and the preparation of oligonucleotide maps is reported. The polypeptide patterns were shown to be a relatively stable property of virus strains and distinguished Sabin vaccine strains from wild strains of poliovirus type 3. This approach may be of value in deciding the origin (vaccine or nonvaccine) of field isolates of poliovirus. Oligonucleotide maps were found to be sensitive indicators of differences among strains and appear to form a basis for determining genetic relationships among strains. The nucleotide maps of two viruses isolated from human cases of paralytic poliomyelitis temporally associated with the administration of attenuated vaccine suggested a vaccine origin for the strain. In one case the nucleotide map was indistinguishable from that of the vaccine strain.
报道了一项关于3型脊髓灰质炎病毒株生物化学的研究，该研究涉及对感染细胞中病毒编码的多肽进行检测以及制备寡核苷酸图谱。结果表明，多肽图谱是病毒株相对稳定的特性，可将萨宾疫苗株与3型脊髓灰质炎病毒野生株区分开来。这种方法在确定脊髓灰质炎病毒现场分离株的来源（疫苗株或非疫苗株）方面可能具有价值。发现寡核苷酸图谱是菌株间差异的敏感指标，似乎构成了确定菌株间遗传关系的基础。从与减毒疫苗接种时间相关的人类麻痹性脊髓灰质炎病例中分离出的两种病毒的核苷酸图谱表明该菌株来源于疫苗。在一个病例中，核苷酸图谱与疫苗株的图谱无法区分。
