Kleinschmidt A M, Martinson H G
Nucleic Acids Res. 1981 Jun 11;9(11):2423-31. doi: 10.1093/nar/9.11.2423.
We have purified uH2A (A24) and reconstituted it, in place of H2A, into high molecular weight nucleohistone containing the core histones and DNA. uH2A-containing core particles were then prepared by nuclease digestion and studies on these particles were carried out. We show that two uH2A molecules can be accommodated within a core particle. We also show that the presence of two uH2A molecules in a core particle does not alter significantly either the pattern or the rate of DNase I digestion as compared to both reconstituted and native core particles. Finally, we show that HMG proteins 14 and 17 can bind to uH2A-containing core particles. We conclude that uH2A has little influence on structure at the level of individual nucleosomes.
我们已经纯化了uH2A（A24），并将其代替H2A重新组装到含有核心组蛋白和DNA的高分子量核组蛋白中。然后通过核酸酶消化制备了含uH2A的核心颗粒，并对这些颗粒进行了研究。我们发现一个核心颗粒中可以容纳两个uH2A分子。我们还表明，与重组核心颗粒和天然核心颗粒相比，核心颗粒中两个uH2A分子的存在不会显著改变DNase I消化的模式或速率。最后，我们表明HMG蛋白14和17可以结合到含uH2A的核心颗粒上。我们得出结论，uH2A在单个核小体水平上对结构影响很小。
