Balàzs E, Guilley H, Jonard G, Richards K
Gene. 1982 Oct;19(3):239-49. doi: 10.1016/0378-1119(82)90013-0.
The double-stranded DNA from the isolate D/H with an altered virulence of the cauliflower mosaic virus (CaMV) contains 8016 bp. The DNA is circular and possesses, like the DNA of most CaMV strains, three sequence interruptions. The comparison of its sequence with the previously published sequences of two other CaMV strains (Cabb-S and CM 1841) leads to the following conclusions: (1) The genetic organization of all three CaMV strains is identical with six potential genes (open reading frames) and two intergenic regions; (2) considered pairwise, the three DNAs differ from one another by only about 5% with base substitutions accounting for most of the changes although several deletions and insertions are also observed. The sequence differences among the three strains are spread in a uniform manner upon the genome except for the two intergenic regions, which are more highly conserved. The stability of the noncoding regions is probably linked to the fact that they carry sequences important for the initiation and termination of transcription. On the other hand, the sequence variation in the open reading frames has relatively little effect on the sequence of the corresponding polypeptides as changes occur preferentially in the third position of the reading frame triplets. It is anticipated that knowledge of the DNA sequences of several CaMV strains will facilitate construction of inter-strain recombinants which, once available, can be used to correlate gene structure and function.
来自具有改变的花椰菜花叶病毒（CaMV）毒力的分离株D/H的双链DNA包含8016个碱基对。该DNA是环状的，并且与大多数CaMV株系的DNA一样，具有三个序列中断。将其序列与另外两个CaMV株系（Cabb-S和CM 1841）先前发表的序列进行比较，得出以下结论：（1）所有三个CaMV株系的基因组织相同，具有六个潜在基因（开放阅读框）和两个基因间区域；（2）两两比较时，这三个DNA彼此之间仅相差约5%，碱基替换占大多数变化，尽管也观察到一些缺失和插入。除了两个基因间区域更为保守外，这三个株系之间的序列差异以均匀的方式分布在基因组上。非编码区的稳定性可能与它们携带对转录起始和终止重要的序列这一事实有关。另一方面，开放阅读框中的序列变异对相应多肽的序列影响相对较小，因为变化优先发生在阅读框三联体的第三位。预计了解几种CaMV株系的DNA序列将有助于构建株系间重组体，一旦获得，可用于关联基因结构和功能。
