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基于质谱的脂质组学:概述。

Mass Spectrometry-Based Lipidomics: An Overview.

机构信息

Mass Spectrometry Resource, Division of Endocrinology, Metabolism, and Lipid Research, Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA.

出版信息

Methods Mol Biol. 2021;2306:1-10. doi: 10.1007/978-1-0716-1410-5_1.

Abstract

Over the last few decades, MS-based lipidomics has emerged as a powerful tool to study lipids in biological systems. This success is driven by the constant demand for complete and reliable data. The improvement of MS-based lipidomics will continue to be dependent on the advances in the technology of mass spectrometry and related techniques including separation and bioinformatics, and more importantly, on gaining insight into the knowledge of lipid chemistry essential to develop methodology for lipid analysis. It is hoped that the protocols in this book, collected from experts in their fields, can offer the beginner and the advanced user alike, useful tips toward successful lipidomic analysis.

摘要

在过去的几十年中,基于 MS 的脂质组学已成为研究生物系统中脂质的有力工具。这种成功是由对完整和可靠数据的不断需求所驱动的。基于 MS 的脂质组学的改进将继续依赖于质谱技术及其相关技术(包括分离和生物信息学)的进步,更重要的是,依赖于深入了解脂质化学知识,这对于开发脂质分析方法至关重要。希望本书中收集的来自各领域专家的方案能够为初学者和高级用户提供成功进行脂质组学分析的有用技巧。

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