Wiskocil R, Goldman P, Deeley R G
J Biol Chem. 1981 Sep 25;256(18):9662-7.
ApoVLDLII is a major polypeptide component of avian very low density lipoprotein. Its production in the liver of the maturing female chick is developmentally synchronized with vitellogenesis. The apoVLDLII gene is normally dormant in males but can be activated by the administration of estrogen. In these studies, we describe the isolation of three recombinant bacteriophages that contain apoVLDLII genes. The genes appear to be identical from preliminary restriction analyses, although heterogeneity in flanking sequences is evident. The structure of the gene has been characterized and its organization correlated with the structure of the apoVLDLII polypeptide.
载脂蛋白VLDLII是禽类极低密度脂蛋白的主要多肽成分。其在成熟雌性雏鸡肝脏中的产生与卵黄生成在发育上同步。载脂蛋白VLDLII基因在雄性中通常处于休眠状态，但可通过给予雌激素而被激活。在这些研究中，我们描述了三种含有载脂蛋白VLDLII基因的重组噬菌体的分离。尽管侧翼序列存在明显的异质性，但从初步的限制性分析来看，这些基因似乎是相同的。该基因的结构已被表征，并且其组织与载脂蛋白VLDLII多肽的结构相关。
