Ferguson M, Schild G C, Minor P D, Yates P J, Spitz M
J Gen Virol. 1981 Jun;54(Pt 2):437-42. doi: 10.1099/0022-1317-54-2-437.
A mouse hybridoma cell line (Mo56) secreting IgG antibody to poliovirus type 3 D-antigen was obtained by fusion of a mouse myeloma cell line with spleen cells from mice immunized with Saukett virus. The monoclonal antibody was specific for Saukett virus strains in virus-neutralization and single-radial-diffusion tests. In immunoprecipitation tests the monoclonal antibody reacted with intact infectious virus particles (155S) and with a previously undescribed 70S poliovirus particle with D-antigenic reactivity.
通过将小鼠骨髓瘤细胞系与用索基特病毒免疫的小鼠脾细胞融合，获得了一种分泌针对脊髓灰质炎病毒3型D抗原的IgG抗体的小鼠杂交瘤细胞系（Mo56）。在病毒中和试验和单向辐射扩散试验中，该单克隆抗体对索基特病毒株具有特异性。在免疫沉淀试验中，该单克隆抗体与完整的感染性病毒颗粒（155S）以及一种以前未描述过的具有D抗原反应性的70S脊髓灰质炎病毒颗粒发生反应。
