纤细裸藻核糖体DNA的特征分析
Characterization of the nuclear ribosomal DNA of Euglena gracilis.
作者信息
Curtis S E, Rawson J R
出版信息
Gene. 1981 Nov;15(2-3):237-47. doi: 10.1016/0378-1119(81)90133-5.
PMID:6271644
Abstract
A phage lambda recombinant library containing Euglena gracilis genomic DNA was screened for nuclear rDNA sequences. A recombinant phage was isolated that contained an 11.5-kb nuclear rDNA sequence. The 11.5-kb insert was mapped with restriction endonucleases and was shown to represent a complete rDNA repeat unit that carried the genes for the 19S, 25S, 5.8 S and 5 S cytoplasmic rRNAs. The 2000 rDNA repeat units per haploid genome are organized in the form of identical tandem repeats.
摘要
利用含有纤细裸藻基因组DNA的λ噬菌体重组文库筛选核rDNA序列。分离出一个含有11.5kb核rDNA序列的重组噬菌体。用限制性内切酶对11.5kb的插入片段进行图谱分析，结果表明它代表一个完整的rDNA重复单元，该单元携带了19S、25S、5.8S和5S细胞质rRNA的基因。单倍体基因组中的2000个rDNA重复单元以相同串联重复的形式排列。
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