珍珠粟叶绿体基因组的核糖体RNA基因和短单拷贝DNA序列的限制酶切图谱。

A restriction map of the ribosomal RNA genes and the short single-copy DNA sequence of the pearl millet chloroplast genome.

作者信息

Rawson J R, Clegg M T, Thomas K, Rinehart C, Wood B

出版信息

Gene. 1981 Dec;16(1-3):11-9. doi: 10.1016/0378-1119(81)90056-1.

Abstract

The chloroplast rDNA genes of pearl millet (Pennisetum americanum) have been cloned and physically mapped. The chloroplast genome of the pearl millet contains two identical rRNA genes located on DNA sequences that are inverted with respect to one another and separated by 12 kb of single-copy DNA. The rRNA genes were positioned on a restriction endonuclease map by using as hybridization probes specific cloned rDNA sequences from the chloroplast DNA of the alga Euglena gracilis. The 16S and 23S rRNA genes were shown to be approx. 2 kb from one another, and the 5S RNA gene is immediately adjacent to the 23S tRNA gene.

摘要

珍珠粟（Pennisetum americanum）的叶绿体rDNA基因已被克隆并进行了物理图谱绘制。珍珠粟的叶绿体基因组包含两个相同的rRNA基因，它们位于彼此反向的DNA序列上，中间相隔12 kb的单拷贝DNA。通过使用来自纤细裸藻叶绿体DNA的特定克隆rDNA序列作为杂交探针，将rRNA基因定位在限制性内切酶图谱上。结果表明，16S和23S rRNA基因彼此相距约2 kb，5S RNA基因紧邻23S tRNA基因。