Pasantes-Morales H, Ademe R M, Quesada O
J Neurosci Res. 1981;6(3):337-48. doi: 10.1002/jnr.490060309.
Isolated frog rod outer segments (ROS) incubated in a Krebs-bicarbonate medium, and illuminated for 2 h, show a profound alteration in their structure. This is characterized by distention of discs, vesiculation, and a marked swelling. The light-induced ROS disruption requires the presence of bicarbonate and sodium chloride. Replacement of bicarbonate by TRIS or HEPES protects ROS structure. Also, substitution of sodium chloride by sucrose or choline chloride maintains unaltered the ROS structure. Deletion of calcium, magnesium, or phosphate does not modify the effect produced by illumination. An increased accumulation of labeled bicarbonate and tritiated water is observed in illuminated ROS, as compared with controls in the dark. The presence of taurine, GABA, or glycine, at concentrations of 5-25 mM, effectively counteracts the light-induced ROS disruption. Taurine (25 mM) reduces labeled bicarbonate and tritiated water levels to those observed in the dark incubated ROS.
在 Krebs - 碳酸氢盐培养基中孵育并光照 2 小时的离体青蛙视杆细胞外段（ROS），其结构会发生显著改变。其特征为盘膜膨胀、形成囊泡以及明显肿胀。光诱导的 ROS 破坏需要碳酸氢盐和氯化钠的存在。用 TRIS 或 HEPES 替代碳酸氢盐可保护 ROS 结构。此外，用蔗糖或氯化胆碱替代氯化钠可使 ROS 结构保持不变。去除钙、镁或磷酸盐不会改变光照产生的效应。与黑暗中的对照相比，光照的 ROS 中观察到标记碳酸氢盐和氚化水的积累增加。浓度为 5 - 25 mM 的牛磺酸、GABA 或甘氨酸的存在可有效对抗光诱导的 ROS 破坏。牛磺酸（25 mM）可将标记碳酸氢盐和氚化水水平降低至黑暗孵育的 ROS 中观察到的水平。
