Grindley J N, Nakada D
Nucleic Acids Res. 1981 Sep 11;9(17):4355-66. doi: 10.1093/nar/9.17.4355.
The sequence of the DNA of the origin region of NTP1 has been obtained. Analysis of the sequence indicates that: (1) there is great sequence homology in the DNA upstream from the origin in NTP1, ColE1, CLODF13, PBR345 AND PBR322; (2) only seven base pairs of NTP1 are identical with the sequence downstream from the origin in ColE1, although some homology exists for 140 bases downstream; (3) two ten base pair direct repeats are present in NTP1 which are also conserved in all four plasmids named above; (4) probably no polypeptide greater than fifteen amino acids in length is encoded by the NTP1 origin region, since no single open reading frame is conserved in all five plasmids.
已获得NTP1起始区域的DNA序列。对该序列的分析表明：（1）NTP1、ColE1、CLODF13、PBR345和PBR322中起始位点上游的DNA存在高度序列同源性；（2）NTP1只有七个碱基对与ColE1中起始位点下游的序列相同，尽管下游140个碱基存在一些同源性；（3）NTP1中存在两个十碱基对的正向重复序列，在上述所有四种质粒中也保守存在；（4）NTP1起始区域可能不编码长度大于十五个氨基酸的多肽，因为在所有五种质粒中没有单一的开放阅读框是保守的。
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