Dotto G P, Enea V, Zinder N D
Proc Natl Acad Sci U S A. 1981 Sep;78(9):5421-24. doi: 10.1073/pnas.78.9.5421.
Plasmids harboring the amino-terminal part of bacteriophage f1 gene II confer to bacterial cells partial resistance to infection with the male-specific bacteriophages f1 and f2. This effect (IP-2 phenotype) is due to the production of large amounts of an approximately 20,000-dalton polypeptide corresponding to the amino-terminal part of gene II protein. These results have allowed the isolation of clones producing functional gene II protein in large amounts. An in vitro assay has been developed to test the enzymatic activity of the gene II protein produced.
携带噬菌体f1基因II氨基末端部分的质粒赋予细菌细胞对雄性特异性噬菌体f1和f2感染的部分抗性。这种效应（IP-2表型）是由于产生了大量对应于基因II蛋白氨基末端部分的约20,000道尔顿的多肽。这些结果使得能够分离出大量产生功能性基因II蛋白的克隆。已经开发出一种体外测定法来测试所产生的基因II蛋白的酶活性。
