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黏粒：一种质粒基因克隆载体，可在体外包装到噬菌体λ头部。

Cosmids: a type of plasmid gene-cloning vector that is packageable in vitro in bacteriophage lambda heads.

作者信息

Collins J, Hohn B

出版信息

Proc Natl Acad Sci U S A. 1978 Sep;75(9):4242-6. doi: 10.1073/pnas.75.9.4242.

DOI:10.1073/pnas.75.9.4242
PMID:360212
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC336088/
Abstract

Evidence is presented that ColE1 hybrid plasmids carrying the cohesive-end site (cos) of lambda can be used as gene cloning vectors in conjunction with the lambda in vitro packaging system of Hohn and Murray [(1977) Proc. Natl. Acad. Sci. USA 74, 3259--3263]. Due to the requirement for a large DNA molecule for efficient packaging, there is a direct selection for hybrids carrying large sections of foreign DNA. The small vector plasmids do not contribute a large background in the transduced population, which is therefore markedly enriched for large hybrid plasmids (over 90%). The efficiency of the in vitro packaging system is on the order of 10(5) hybrid clones per microgram of foreign DNA for hybrids in the 20--30 million dalton range.

摘要

有证据表明，携带λ噬菌体粘性末端位点（cos）的ColE1杂种质粒可与霍恩和默里的λ噬菌体体外包装系统[（1977年）《美国国家科学院院刊》74, 3259 - 3263]一起用作基因克隆载体。由于高效包装需要大的DNA分子，因此可直接筛选携带大片段外源DNA的杂种。小载体质粒在转导群体中不会产生大量背景，因此转导群体中显著富集了大型杂种质粒（超过90%）。对于2000万至3000万道尔顿范围内的杂种，体外包装系统的效率约为每微克外源DNA产生10⁵个杂种克隆。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4be/336088/0a1c1a11bf02/pnas00668-0183-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4be/336088/0a1c1a11bf02/pnas00668-0183-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4be/336088/0a1c1a11bf02/pnas00668-0183-a.jpg

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1
Cosmids: a type of plasmid gene-cloning vector that is packageable in vitro in bacteriophage lambda heads.黏粒：一种质粒基因克隆载体，可在体外包装到噬菌体λ头部。
Proc Natl Acad Sci U S A. 1978 Sep;75(9):4242-6. doi: 10.1073/pnas.75.9.4242.
2
Plasmids useable as gene-cloning vectors in an in vitro packaging by coliphage lambda: "cosmids".可用作通过λ噬菌体进行体外包装的基因克隆载体的质粒：“黏粒” 。
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Construction of a lambda packageable ColE1 vector which permits cloning of large DNA fragments: cloning of thyA gene of Escherichia coli.一种可包装入λ噬菌体的ColE1载体的构建，该载体允许克隆大片段DNA：大肠杆菌thyA基因的克隆
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Packaging recombinant DNA molecules into bacteriophage particles in vitro.在体外将重组DNA分子包装到噬菌体颗粒中。
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Construction and some properties of packageable plasmid F.可包装质粒F的构建及其某些特性
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本文引用的文献

1
Activity of empty, headlike particles for packaging of DNA of bacteriophage lambda in vitro.空的、头状颗粒在体外包装噬菌体λ DNA的活性。
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2372-6. doi: 10.1073/pnas.71.6.2372.
2
Bacteriophage lambda derivatives carrying two copies of the cohesive end site.携带两个粘性末端位点拷贝的λ噬菌体衍生物。
J Mol Biol. 1974 Mar 15;83(4):511-25. doi: 10.1016/0022-2836(74)90511-7.
3
In vitro genetic recombination of bacteriophage lambda.噬菌体λ的体外基因重组
工程噬菌体疗法：原理、挑战与未来。
BioDrugs. 2021 May;35(3):255-280. doi: 10.1007/s40259-021-00480-z. Epub 2021 Apr 21.
4
Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts.细胞膜破坏介导的细胞内递送：机制、策略和概念。
Chem Rev. 2018 Aug 22;118(16):7409-7531. doi: 10.1021/acs.chemrev.7b00678. Epub 2018 Jul 27.
5
Monochromosomal Hybrids and Chromosome Transfer: A Functional Approach for Gene Identification.单染色体杂种与染色体转移：一种用于基因鉴定的功能方法。
Cancer Genomics Proteomics. 2017 Mar-Apr;14(2):93-101. doi: 10.21873/cgp.20022.
6
A century of the phage: past, present and future.一个世纪的噬菌体：过去、现在和未来。
Nat Rev Microbiol. 2015 Dec;13(12):777-86. doi: 10.1038/nrmicro3564. Epub 2015 Nov 9.
7
Using linkage maps to correct and scaffold de novo genome assemblies: methods, challenges, and computational tools.利用连锁图谱校正和搭建从头基因组组装：方法、挑战及计算工具
Front Genet. 2015 Jun 19;6:220. doi: 10.3389/fgene.2015.00220. eCollection 2015.
8
Bacteriophage lambda: Early pioneer and still relevant.噬菌体λ：早期先驱且仍具相关性。
Virology. 2015 May;479-480:310-30. doi: 10.1016/j.virol.2015.02.010. Epub 2015 Mar 3.
9
Construction of new yeast vectors and cloning of the nif (nitrogen fixation) gene cluster of Klebsiella pneumoniae in yeast.在酵母中构建新的酵母载体并克隆肺炎克雷伯氏菌的 nif（氮固定）基因簇。
Curr Genet. 1981 Jul;3(3):173-80. doi: 10.1007/BF00429819.
10
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Curr Genet. 1984 May;8(4):291-7. doi: 10.1007/BF00419727.
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2496-9. doi: 10.1073/pnas.71.6.2496.
4
DNA as substrate for packaging into bacteriophage lambda, in vitro.体外将DNA作为包装进噬菌体λ的底物。
J Mol Biol. 1975 Oct 15;98(1):93-106. doi: 10.1016/s0022-2836(75)80103-3.
5
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6
Tandem genetic duplications in phage lambda. III. The frequency of duplication mutants in two derivatives of phage lambda is independent of known recombination systems.噬菌体λ中的串联基因重复。III. 噬菌体λ两个衍生物中重复突变体的频率与已知重组系统无关。
J Mol Biol. 1975 Jan 15;91(2):133-46. doi: 10.1016/0022-2836(75)90154-0.
7
Early events in the in vitro packaging of bacteriophage lambda DNA.噬菌体λ DNA体外包装的早期事件。
Virology. 1977 May 1;78(1):291-305. doi: 10.1016/0042-6822(77)90100-3.
8
Studies on an in vitro system for the packaging and maturation of phage lambda DNA.λ噬菌体DNA包装与成熟的体外系统研究
Virology. 1977 May 1;78(1):277-90. doi: 10.1016/0042-6822(77)90099-x.
9
Packaging of the bacteriophage lambda chromosome: effect of chromosome length.噬菌体λ染色体的包装：染色体长度的影响
Virology. 1977 Mar;77(1):281-93. doi: 10.1016/0042-6822(77)90425-1.
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Cell. 1976 Sep;9(1):91-9. doi: 10.1016/0092-8674(76)90055-6.