Heslop J P, Berridge M J
Biochem J. 1980 Oct 15;192(1):247-55. doi: 10.1042/bj1920247.
Salivary glands from adult blowflies (Calliphora erythrocephala Meigen) were studied in vitro. The time course of changes in cyclic AMP content of the glands was followed at different concentration of 5-hydroxytryptamine. There was an immediate biphasic rise and fall in cyclic AMP content, following by a slower rise and subsequent gradual decline. The initial rise preceded the onset of fluid secretion by the glands. Rises in cyclic AMP content were inhibited by compound RMI 12330 A (an adenylate cyclase inhibitor) and were halted after about 15-20s if the glands were deprived of Ca2+. Theophylline (a phosphodiesterase inhibitor) abolished the decline phase of the fast response, Losses of cyclic AMP from the glands either to the bathing medium or to the saliva were small and could not account for the rapid fall found. Evidence is presented that cyclic GMP is not involved in the process of initiating secretion in the blowfly salivary gland.
对成年丽蝇（红头丽蝇）的唾液腺进行了体外研究。在不同浓度的5-羟色胺作用下，跟踪唾液腺中环状AMP含量的变化时间进程。环状AMP含量立即出现双相上升和下降，随后是较慢的上升以及随后的逐渐下降。最初的上升先于腺体分泌液体的开始。化合物RMI 12330 A（一种腺苷酸环化酶抑制剂）抑制了环状AMP含量的上升，如果腺体缺乏Ca2+，则在约15 - 20秒后上升停止。茶碱（一种磷酸二酯酶抑制剂）消除了快速反应的下降阶段，唾液腺中环状AMP向浴液介质或唾液中的损失很小，无法解释所发现的快速下降。有证据表明，环状GMP不参与丽蝇唾液腺启动分泌的过程。
