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巯基蛋白酶抑制剂对培养中骨吸收的抑制作用。

Inhibition of bone resorption in culture by inhibitors of thiol proteinases.

作者信息

Delaissé J M, Eeckhout Y, Vaes G

出版信息

Biochem J. 1980 Oct 15;192(1):365-8. doi: 10.1042/bj1920365.

DOI:10.1042/bj1920365
PMID:6272702
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1162343/
Abstract

Leupeptin, antipain, tosyl-lysylchloromethane (Tos-Lys-CH2Cl) and benzyloxy-carbonylphenylalanylalanyldiazomethane (Z-Phe-Ala-CHN2) inhibit reversibly the resorption induced by parathyroid hormone or heparin in cultured mouse bones. Leupeptin and antipain do not affect collagenase production and activity or the enhanced secretion of beta-glucuronidase induced by the bone-resorbing agents. They might thus act by a direct (extracellular?) inhibition of lysosomal thiol proteinases.

摘要

亮抑酶肽、抗痛素、甲苯磺酰 - 赖氨酰氯甲烷（Tos - Lys - CH₂Cl）和苄氧羰基苯丙氨酰丙氨酰重氮甲烷（Z - Phe - Ala - CHN₂）可可逆性抑制甲状旁腺激素或肝素在培养的小鼠骨骼中诱导的吸收。亮抑酶肽和抗痛素不影响胶原酶的产生和活性，也不影响骨吸收剂诱导的β - 葡萄糖醛酸酶分泌增加。因此，它们可能通过直接（细胞外？）抑制溶酶体巯基蛋白酶发挥作用。

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Inhibition of bone resorption in culture by inhibitors of thiol proteinases.巯基蛋白酶抑制剂对培养中骨吸收的抑制作用。
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本文引用的文献

1
The inhibition of macrophage protein turnover by a selective inhibitor of thiol proteinases.巯基蛋白酶选择性抑制剂对巨噬细胞蛋白质周转的抑制作用。
Biochem J. 1980 Feb 15;186(2):385-90. doi: 10.1042/bj1860385.
2
On the mechanisms of bone resorption. The action of parathyroid hormone on the excretion and synthesis of lysosomal enzymes and on the extracellular release of acid by bone cells.论骨吸收的机制。甲状旁腺激素对溶酶体酶排泄与合成以及骨细胞酸细胞外释放的作用。
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3
Glycosidases in the nervous system. I. Assay, some properties, and distribution of beta-galactosidase, beta-glucoronidase, and beta-glucosidase.神经系统中的糖苷酶。I. β-半乳糖苷酶、β-葡萄糖醛酸酶和β-葡萄糖苷酶的测定、某些性质及分布
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The release of collagenase as an inactive proenzyme by bone explants in culture.骨外植体在培养过程中以无活性的酶原形式释放胶原酶。
Biochem J. 1972 Jan;126(2):275-89. doi: 10.1042/bj1260275.
5
Excretion of acid and of lysosomal hydrolytic enzymes during bone resorption induced in tissue culture by parathyroid extract.甲状旁腺提取物在组织培养中诱导骨吸收过程中酸和溶酶体水解酶的排泄。
Exp Cell Res. 1965 Sep;39(2):470-4. doi: 10.1016/0014-4827(65)90050-9.
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Cathepsin B1. A lysosomal enzyme that degrades native collagen.组织蛋白酶B1。一种降解天然胶原蛋白的溶酶体酶。
Biochem J. 1974 Feb;137(2):387-98. doi: 10.1042/bj1370387.
7
Purification of bovine spleen collagenolytic cathepsin, (cathepsin N).牛脾脏胶原分解组织蛋白酶（组织蛋白酶N）的纯化
Biochem Soc Trans. 1978;6(5):938-40. doi: 10.1042/bst0060938.
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Collagenase, procollagenase and bone resorption. Effects of heparin, parathyroid hormone and calcitonin.胶原酶、前胶原酶与骨吸收。肝素、甲状旁腺激素及降钙素的作用。
Biochim Biophys Acta. 1979 May 16;584(3):375-88. doi: 10.1016/0304-4165(79)90114-4.
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Proteolytic enzymes, cell surface changes, and viral transformation.蛋白水解酶、细胞表面变化与病毒转化
Adv Cancer Res. 1975;22:203-60. doi: 10.1016/s0065-230x(08)60178-5.