巯基蛋白酶抑制剂对培养中骨吸收的抑制作用。
Inhibition of bone resorption in culture by inhibitors of thiol proteinases.
作者信息
Delaissé J M, Eeckhout Y, Vaes G
出版信息
Biochem J. 1980 Oct 15;192(1):365-8. doi: 10.1042/bj1920365.
Abstract
Leupeptin, antipain, tosyl-lysylchloromethane (Tos-Lys-CH2Cl) and benzyloxy-carbonylphenylalanylalanyldiazomethane (Z-Phe-Ala-CHN2) inhibit reversibly the resorption induced by parathyroid hormone or heparin in cultured mouse bones. Leupeptin and antipain do not affect collagenase production and activity or the enhanced secretion of beta-glucuronidase induced by the bone-resorbing agents. They might thus act by a direct (extracellular?) inhibition of lysosomal thiol proteinases.
摘要
亮抑酶肽、抗痛素、甲苯磺酰 - 赖氨酰氯甲烷（Tos - Lys - CH₂Cl）和苄氧羰基苯丙氨酰丙氨酰重氮甲烷（Z - Phe - Ala - CHN₂）可可逆性抑制甲状旁腺激素或肝素在培养的小鼠骨骼中诱导的吸收。亮抑酶肽和抗痛素不影响胶原酶的产生和活性，也不影响骨吸收剂诱导的β - 葡萄糖醛酸酶分泌增加。因此，它们可能通过直接（细胞外？）抑制溶酶体巯基蛋白酶发挥作用。
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