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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Goto Y, Amuro N, Shukuya R

Biochim Biophys Acta. 1982 Oct 28;719(1):102-9. doi: 10.1016/0304-4165(82)90313-0.

Cytochrome c oxidase was purified from the liver mitochondria of bullfrog (Rana catesbeiana). The heme a content of the purified enzyme was 13.5 nmol per mg protein. Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate revealed that the enzyme protein was composed of nine polypeptide subunits having molecular weights of 42000, 27000, 25000, 20000, 15000, 13000, 8600, 5400 and 3600. The purified enzyme from the adult frog was immunological identified with that from the tadpole. 2. The ratio of synthesis and degradation of cytochrome c oxidase were 5.2- and 2.0-times higher at metamorphic climax than at premetamorphic stage, respectively. The amount of the enzyme in the liver was highest at metamorphic climax.

细胞色素c氧化酶是从牛蛙（牛蛙）肝脏线粒体中纯化得到的。纯化酶的血红素a含量为每毫克蛋白质13.5纳摩尔。在十二烷基硫酸钠存在下的聚丙烯酰胺凝胶电泳显示，酶蛋白由九个多肽亚基组成，分子量分别为42000、27000、25000、20000、15000、13000、8600、5400和3600。成年青蛙的纯化酶与蝌蚪的纯化酶进行了免疫鉴定。2. 细胞色素c氧化酶的合成与降解比率在变态高峰期分别比变态前期高5.2倍和2.0倍。肝脏中该酶的含量在变态高峰期最高。

Goto Y, Amuro N, Shukuya R

Biochim Biophys Acta. 1982 Oct 28;719(1):102-9. doi: 10.1016/0304-4165(82)90313-0.

Cytochrome c oxidase was purified from the liver mitochondria of bullfrog (Rana catesbeiana). The heme a content of the purified enzyme was 13.5 nmol per mg protein. Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate revealed that the enzyme protein was composed of nine polypeptide subunits having molecular weights of 42000, 27000, 25000, 20000, 15000, 13000, 8600, 5400 and 3600. The purified enzyme from the adult frog was immunological identified with that from the tadpole. 2. The ratio of synthesis and degradation of cytochrome c oxidase were 5.2- and 2.0-times higher at metamorphic climax than at premetamorphic stage, respectively. The amount of the enzyme in the liver was highest at metamorphic climax.

细胞色素c氧化酶是从牛蛙（牛蛙）肝脏线粒体中纯化得到的。纯化酶的血红素a含量为每毫克蛋白质13.5纳摩尔。在十二烷基硫酸钠存在下的聚丙烯酰胺凝胶电泳显示，酶蛋白由九个多肽亚基组成，分子量分别为42000、27000、25000、20000、15000、13000、8600、5400和3600。成年青蛙的纯化酶与蝌蚪的纯化酶进行了免疫鉴定。2. 细胞色素c氧化酶的合成与降解比率在变态高峰期分别比变态前期高5.2倍和2.0倍。肝脏中该酶的含量在变态高峰期最高。