Kmiec E, Holloman W K
J Biol Chem. 1981 Dec 25;256(24):12636-9.
The protein encoded by the red beta gene of bacteriophage lambda was found to promote reannealing of complementary single strands of DNA. Reannealing activity was optimal at pH 6.0 and required a divalent cation. A threshold temperature of at least 15 degrees C was necessary in order to detect activity. The reaction was linear with time for about 20 min, but the extent of reaction was dependent upon the amount of beta protein added. Reannealing of complementary single strands was confirmed by measuring increased lability of DNA to lambda exonuclease. When protein preparations from red- lysogens were tested for ability to promote reannealing, high activity was observed in a mutant altered in the gene for exonuclease, but there was low activity in a mutant altered in the gene for beta protein.
发现噬菌体λ的红色β基因编码的蛋白质可促进DNA互补单链的复性。复性活性在pH 6.0时最佳，且需要二价阳离子。为了检测活性，至少15℃的阈值温度是必要的。反应在约20分钟内与时间呈线性关系，但反应程度取决于添加的β蛋白的量。通过测量DNA对λ核酸外切酶的敏感性增加来确认互补单链的复性。当测试来自红色溶原菌的蛋白质制剂促进复性的能力时，在核酸外切酶基因发生改变的突变体中观察到高活性，但在β蛋白基因发生改变的突变体中活性较低。
