Gaub J
Histochemistry. 1976 Oct 22;49(2):113-21. doi: 10.1007/BF00495675.
From rats fed ad libitum and kept under a 12 + 12 h light/dark regimen, the DNA dependent RNA polymerase activity of liver cell nuclei was determined avery four hours. From identical rats, nuclear non-histone protein and DNA, and cytoplasmic protein was determined by Feulgen-Naphthol Yellow S cytophotometry of isolated liver cells. The minimum: maximum ratio of the RNA polymerase activity is 0.77; the min:max ratio of nuclear non-histone protein is 0.84. These two parameters have identical time courses with a gradual decline during the light period and a sharp rise after the onset of the dark period. The variations in nuclear DNA content, estimated as the amount of Feulgen stain bound, closely parallel those of the RNA polymerase activity and nuclear non-histone protein content (min:max = 0.96). The amount of cytoplasmic protein per cell also varies throughout the day, but its time curve lags behind those of nuclear non-histone content and RNA polymerase activity. These results are consistent with the concept of nuclear non-histone proteins as de-repressors of the DNA template in differentiated, non-proliferating cells, and support the validity of using Feulgen-Naphthol Yellow S cytophotometry of nuclear non-histone proteins as an estimate of gene expression in such cells.
从自由采食并饲养在12小时光照/12小时黑暗周期条件下的大鼠中，每4小时测定一次肝细胞细胞核的DNA依赖性RNA聚合酶活性。从相同的大鼠中，通过对分离的肝细胞进行Feulgen-萘酚黄S细胞光度测定法来测定细胞核非组蛋白、DNA以及细胞质蛋白。RNA聚合酶活性的最低值与最高值之比为0.77；细胞核非组蛋白的最低值与最高值之比为0.84。这两个参数具有相同的时间进程，在光照期逐渐下降，在黑暗期开始后急剧上升。以结合的Feulgen染色量估算的细胞核DNA含量变化与RNA聚合酶活性和细胞核非组蛋白含量的变化密切平行（最低值与最高值之比=0.96）。每个细胞的细胞质蛋白量在一天中也会变化，但其时间曲线滞后于细胞核非组蛋白含量和RNA聚合酶活性的时间曲线。这些结果与细胞核非组蛋白作为分化的、非增殖细胞中DNA模板去阻遏物的概念一致，并支持使用Feulgen-萘酚黄S细胞光度测定法测定细胞核非组蛋白作为此类细胞中基因表达的一种估计方法的有效性。
