Frederiks W M, Slob A, Schröder M
Histochemistry. 1980;68(1):49-53. doi: 10.1007/BF00498500.
The purpose of this study is to compare the protein content of parenchymal and non-parenchymal nuclei, as isolated from rat liver. The nuclei have been separated by means of a 1 g-sedimentation technique. The protein content of the separated nuclei has been determined cytophotometrically using the Naphthol Yellow S staining procedure after TCA-extraction (corresponding with the total protein content) and directly (corresponding with the non-histone proteins). The ratio of the total protein content of non-parenchymal, parenchymal diploid and parenchymal tetraploid nuclei respectively was found to be 0.65:100:1.90. The ratio of non-histone protein a total protein was the same for all types of nuclei investigated, namely about 55%.
本研究的目的是比较从大鼠肝脏分离出的实质细胞核与非实质细胞核的蛋白质含量。细胞核已通过1g沉降技术进行分离。分离出的细胞核的蛋白质含量已在三氯乙酸提取后（对应总蛋白质含量）使用萘酚黄S染色程序通过细胞光度测定法进行测定，并直接测定（对应非组蛋白）。非实质细胞核、实质二倍体细胞核和实质四倍体细胞核的总蛋白质含量之比分别为0.65:100:1.90。在所研究的所有类型的细胞核中，非组蛋白与总蛋白的比例相同，约为55%。
