Jackson V, Chalkley R
Proc Natl Acad Sci U S A. 1981 Oct;78(10):6081-5. doi: 10.1073/pnas.78.10.6081.
Formaldehyde fixation of simian virus 40 (SV40)-infected CV-1 cells at appropriate times after infection permits us to isolate crosslinked complexes of SV40 minichromosomes during the time of DNA replication and during packaging with viral proteins. Such crosslinked complexes can be separated on the basis of density on CsCl/guanidine . HCl density gradients. During the course of these studies we observed the presence of a low molecular weight protein in a region of the gradient much enriched with viral nucleoproteins. This protein is present only in infected cells and has a molecular weight and amino acid composition consistent with it being the product of the so-called SV40 agnogene.
在感染猿猴病毒40（SV40）的CV - 1细胞感染后的适当时间进行甲醛固定，这使我们能够在DNA复制期间以及与病毒蛋白包装过程中分离出SV40微型染色体的交联复合物。这种交联复合物可以根据其在CsCl/盐酸胍密度梯度上的密度进行分离。在这些研究过程中，我们在梯度中一个富含病毒核蛋白的区域观察到一种低分子量蛋白质的存在。这种蛋白质仅存在于受感染的细胞中，其分子量和氨基酸组成与所谓的SV40辅助基因的产物一致。
