Hammonds R G, Li C H
Proc Natl Acad Sci U S A. 1981 Nov;78(11):6764-5. doi: 10.1073/pnas.78.11.6764.
The mouse neuroblastoma N18TG2 has specific, saturable binding sites for human beta-endorphin (beta h-EP). The affinity and number of sites are 1.1 nM and 280,000 per cell, respectively, beta h-EP binding is not inhibited by [Leu]enkephalin or morphine at concentrations up to 0.1 mM; beta h-EP-(6--31) is a potent inhibitor of binding, and camel beta-EP is much less potent. The data suggest the importance of the nonenkephalin segment of the beta h-EP molecule for interaction with the binding site in N18TG2 cells.
小鼠神经母细胞瘤N18TG2对人β-内啡肽（βh-EP）具有特异性的、可饱和的结合位点。其结合位点的亲和力和数量分别为1.1 nM和每个细胞280,000个。在浓度高达0.1 mM时，[亮氨酸]脑啡肽或吗啡不会抑制βh-EP的结合；βh-EP-(6-31)是一种有效的结合抑制剂，而骆驼β-内啡肽的抑制作用则弱得多。这些数据表明βh-EP分子的非脑啡肽片段对于与N18TG2细胞中的结合位点相互作用很重要。
