Asakura K, Kataura A
Acta Otolaryngol. 1981 Jul-Aug;92(1-2):181-8. doi: 10.3109/00016488109133254.
cAMP metabolism, including the intracellular cAMP level and adenylcyclase activity, was studied in tonsillar and peripheral lymphocytes. The basal activity of adenylcyclase was significantly higher in tonsillar lymphocytes than in peripheral lymphocytes, although the basal level of intracellular cAMP did not differ. The responsiveness of the intracellular cAMP level and adenylcyclase activity stimulated by 10(-5) M isoproterenol, or 10(-5) M prostaglandin E1, which was thought to stimulate this enzyme through a specific receptor, was significantly lower in tonsillar lymphocytes than in peripheral lymphocytes. Moreover, the responsiveness of adenylcyclase activity to 10(-2) M NaF, which was thought to stimulate this enzyme directly, was also significantly lower in tonsillar lymphocytes. Tonsillar T lymphocytes represented a lower cAMP basal level than tonsillar B lymphocytes, but no difference was observed in the responsiveness of intracellular cAMP to the above agents. Tonsillar lymphocytes derived from patients with recurrent tonsillitis represented significantly lower cAMP responsiveness than those from patients of other chronic tonsillitis. Furthermore, in the tonsillar lymphocytes from patients with a recent attack of acute tonsillitis, a more depressed responsiveness of the intracellular cAMP level to the above agents was recognized. The cAMP basal level was not changed by these pathological alterations of tonsillitis.
在扁桃体淋巴细胞和外周淋巴细胞中研究了环磷酸腺苷（cAMP）代谢，包括细胞内cAMP水平和腺苷酸环化酶活性。尽管细胞内cAMP的基础水平没有差异，但扁桃体淋巴细胞中腺苷酸环化酶的基础活性显著高于外周淋巴细胞。10^(-5)M异丙肾上腺素或10^(-5)M前列腺素E1（被认为通过特定受体刺激该酶）刺激后，扁桃体淋巴细胞中细胞内cAMP水平和腺苷酸环化酶活性的反应性显著低于外周淋巴细胞。此外，10^(-2)M氟化钠（被认为直接刺激该酶）刺激后，扁桃体淋巴细胞中腺苷酸环化酶活性的反应性也显著降低。扁桃体T淋巴细胞的cAMP基础水平低于扁桃体B淋巴细胞，但细胞内cAMP对上述试剂的反应性未观察到差异。复发性扁桃体炎患者的扁桃体淋巴细胞的cAMP反应性显著低于其他慢性扁桃体炎患者。此外，在近期患有急性扁桃体炎发作的患者的扁桃体淋巴细胞中，细胞内cAMP水平对上述试剂的反应性更为降低。这些扁桃体炎的病理改变并未改变cAMP基础水平。
