Wiseman G M, McNicol P, Lian C J, Primrose D S
Can J Microbiol. 1981 Oct;27(10):1035-43. doi: 10.1139/m81-161.
The nature of the receptors for T1 and T4 Neisseria gonorrhoeae on erythrocytes and other cells was investigated. In general, cells of nonprimate origin contained few receptors for gonococci. Receptors for T4 gonococci were only uncovered when host cells were pretreated with trypsin. Trypsinization, while unnecessary for T1 adherence to erythrocytes, enhanced attachment in inverse proportion to original erythrocyte sensitivity. Receptors for T1 and T4 organisms on trypsinized and trypsin-neuraminidase-treated erythrocytes were blocked by concanavalin A and peanut lectins, respectively, but a distinction could be made between them with wheat germ lectin and galactose oxidase. Of a number of sugars tested as inhibitors, only D-galactose blocked adherence of T4 but was without effect on T1. While the identity of erythrocyte receptors is uncertain, likely candidates are "band 3" protein and glycophorin, by virtue of their galactose content, lectin binding capacity, and partial exposure on the outer surface of the erythrocyte.
研究了淋病奈瑟菌T1和T4型在红细胞及其他细胞上的受体性质。一般来说，非灵长类来源的细胞含有的淋球菌受体很少。只有当宿主细胞用胰蛋白酶预处理后，T4淋球菌的受体才会显现出来。胰蛋白酶处理对T1黏附于红细胞并非必需，但会增强其黏附，且与红细胞的原始敏感性成反比。经胰蛋白酶处理和胰蛋白酶-神经氨酸酶处理的红细胞上，T1和T4菌株的受体分别被伴刀豆球蛋白A和花生凝集素阻断，但用小麦胚凝集素和半乳糖氧化酶可区分它们。在作为抑制剂测试的多种糖类中，只有D-半乳糖能阻断T4的黏附，但对T1无作用。虽然红细胞受体的身份尚不确定，但“带3”蛋白和血型糖蛋白因其半乳糖含量、凝集素结合能力以及在红细胞外表面的部分暴露，可能是受体的候选者。
