Wiseman G M, McNicol P
Can J Microbiol. 1982 Feb;28(2):219-22. doi: 10.1139/m82-029.
Normal and trypsinized human erythrocyte membranes were used as a model in the study of host cell receptors for Neisseria gonorrhoeae. Receptor sites were identified by adherence inhibition assays of fractions of membranes eluted from polyacrylamide gel electrophoresis columns. Results indicated that inhibition of gonococcus T1 and T4 adherence was associated with erythrocyte protein band 3 and 4 and glycophorin A, the major sialoglycoprotein. Further investigation revealed that band 3 preparations isolated by affinity chromatography on concanavalin A - Sepharose 4B columns continued to inhibit T1 adherence to erythrocytes but did not inhibit adherence of T4 organisms. It is suggested that protein band 3 is the receptor on erythrocytes for T1 gonococci and that glycophorin A may be the receptor for T4 cells.
在淋病奈瑟菌宿主细胞受体的研究中，使用正常的和经胰蛋白酶处理的人红细胞膜作为模型。通过对从聚丙烯酰胺凝胶电泳柱洗脱的膜组分进行黏附抑制试验来鉴定受体位点。结果表明，淋球菌T1和T4的黏附抑制与红细胞蛋白带3、4以及主要的唾液酸糖蛋白血型糖蛋白A有关。进一步研究发现，通过伴刀豆球蛋白A - 琼脂糖4B柱亲和层析分离得到的带3制剂仍能抑制T1对红细胞的黏附，但不能抑制T4菌株的黏附。提示蛋白带3是红细胞上T1淋球菌的受体，而血型糖蛋白A可能是T4细胞的受体。
