Allen P Z, Connelly M C, Apicella M A
Can J Microbiol. 1980 Apr;26(4):468-74. doi: 10.1139/m80-078.
Fifty-four strains of Neisseria gonorrhoeae were examined for their ability to be agglutinated by various lectins. Wheat germ agglutinin, ricin, soybean lectin, and peanut agglutinin agglutinated all strains tested. Dolichos biflorus, Sophora japonica, Maclura pomifera, Ulex Europaeus, Lens culinaris, Canavalia ensiformis, Phaseolus lunatus, and Bandeiraea simplicifolia BS I and BS II lectins either failed to agglutinate or agglutinated some strains but not others. Agglutination of gonococci by wheat germ agglutinin, ricin, and soybean lectin is sugar specific and most effectively inhibited by ligands known to interact with lectin combining sites. Lectin reactive groupings appear to be independent of antigenic determinants conferring Gc serogroup specificity. Interactions with wheat germ agglutinin and ricin suggest the occurrence of multiple beta-linked N-acetyl-D-glucosamine (D-GlcNAc) and beta-D-galactosyl (beta-D-Gal) units as common structural features of gonococcal cell envelope polysaccharide. Interactions with soybean agglutinin, peanut agglutinin, and Dolichos biflorus lectins suggest that alpha-N-acetyl-D-galactosamine (alpha-D-GalNAc) units and beta-D-Gal linked to GalNAc and (or) GlcNAc may also occur as structural features of the polysaccharide components of the cell envelope of some gonococci.
检测了54株淋病奈瑟菌被各种凝集素凝集的能力。麦胚凝集素、蓖麻毒素、大豆凝集素和花生凝集素凝集了所有测试菌株。双花扁豆、槐、桑橙、荆豆、兵豆、刀豆、利马豆以及单叶豆凝集素BS I和BS II要么不能凝集，要么只能凝集部分菌株而不能凝集其他菌株。麦胚凝集素、蓖麻毒素和大豆凝集素对淋球菌的凝集具有糖特异性，并且能被已知与凝集素结合位点相互作用的配体最有效地抑制。凝集素反应基团似乎独立于赋予Gc血清群特异性的抗原决定簇。与麦胚凝集素和蓖麻毒素的相互作用表明，多个β-连接的N-乙酰-D-葡萄糖胺（D-GlcNAc）和β-D-半乳糖基（β-D-Gal）单元作为淋球菌细胞壁多糖的常见结构特征存在。与大豆凝集素、花生凝集素和双花扁豆凝集素的相互作用表明，α-N-乙酰-D-半乳糖胺（α-D-GalNAc）单元以及与GalNAc和（或）GlcNAc相连的β-D-Gal也可能作为某些淋球菌细胞壁多糖成分的结构特征出现。
