Sheppard A M, Smith J W
Curr Eye Res. 1981;1(7):397-402. doi: 10.3109/02713688109019977.
The ability of complement-dependent antibody to mediate destruction of rabbit corneal cells infected with the RE and KOS strains of HSV-1 was examined. Three sources of target cells were used: BHK-21, a monitor of surface antigen expression and cell lysis; SIRC, a continuous cell line of rabbit corneal origin; stromal keratocytes, grown from the middle layer of excised rabbit corneas. Rabbits were infected intrastromally with the RE strain, which causes a high incidence of stromal keratitis, and sacrificed at designated times. Results showed that all three cell types, infected with either HSV strain, expressed surface antigens and were susceptible to lysis by antibody produced during the course of stromal disease. 51Cr-release assays showed that cytolytic antibody appeared as early as 5 days postinfection and reached maximum by day 14-20. Findings suggest a direct relationship between the in vitro demonstration of complement dependent cytolysis of infected stromal cells and the disease process in experimental animals.
研究了补体依赖性抗体介导对感染单纯疱疹病毒1型（HSV-1）RE株和KOS株的兔角膜细胞破坏的能力。使用了三种来源的靶细胞：BHK-21，一种表面抗原表达和细胞裂解的监测细胞；SIRC，一种源自兔角膜的连续细胞系；从切除的兔角膜中层培养的基质角膜细胞。兔经基质内感染RE株（该毒株可导致基质性角膜炎的高发病率），并在指定时间处死。结果显示，感染任一HSV毒株的所有三种细胞类型均表达表面抗原，并且易受基质疾病过程中产生的抗体的裂解作用。51Cr释放试验表明，溶细胞抗体最早在感染后5天出现，并在第14至20天达到最大值。研究结果表明，感染的基质细胞补体依赖性细胞溶解的体外表现与实验动物的疾病过程之间存在直接关系。
