Metcalf J F, Helmsen R
Invest Ophthalmol Vis Sci. 1977 Sep;16(9):779-86.
Sheep antihuman IgG-antiferritin hybrid antibodies were used for the ultrastructural localization of herpes simplex virus (HSV) antigens in rabbit corneas from animals with herpetic keratitis. In animals with epithelial keratitis in which active viral replication is occurring (6 days after infection), viral antigen was found within nuclei, on nuclear membranes, and on cell surface membranes of epithelial cells. In animals with early necrotizing keratitis in which active viral replication cannot be demonstrated (14 to 21 days after infection), viral antigen was found in association with the cell surface of stromal keratocytes. Since lymphocytic cells in intimate contact with degenerating keratocytes have previously been identified in the cornea, these observations provide a basis for the view that cell-mediated immunopathogenesis is involved in the etiology of herpetic stromal keratitis.
羊抗人IgG - 抗铁蛋白杂交抗体被用于对患有疱疹性角膜炎的兔角膜中单纯疱疹病毒（HSV）抗原进行超微结构定位。在发生上皮性角膜炎且有活跃病毒复制的动物（感染后6天）中，病毒抗原见于上皮细胞核内、核膜上以及细胞表面膜上。在患有早期坏死性角膜炎且无法证明有活跃病毒复制的动物（感染后14至21天）中，病毒抗原见于与基质角膜细胞的细胞表面相关处。由于先前已在角膜中鉴定出与变性角膜细胞密切接触的淋巴细胞，这些观察结果为细胞介导的免疫发病机制参与疱疹性基质性角膜炎的病因学这一观点提供了依据。
